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slideview vs200 asw 4 1 imaging system  (Olympus)


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    Structured Review

    Olympus slideview vs200 asw 4 1 imaging system
    Study overview. Carotid bodies from SHR and Wistar controls were analysed across different age groups. The common carotid artery bifurcation (unilateral) was serially sectioned at 20 µm in the sagittal plane, with sections mounted in sequence (roman numerals on a representative slide) to permit reconstruction of rostral–caudal directionality following image acquisition. Multiplex fluorescent labelling targeted key components: chemosensory (glomus) cells (TH—tyrosine hydroxylase), vasculature (TL—tomato lectin), and innervation (NF—160 kDa neurofilament). Imaging was performed using a high-throughput widefield fluorescence microscopy platform (Olympus <t>VS200).</t> Lucy–Richardson deconvolution was applied, and binary area segmentation was performed using an artificial neural network-based pixel classification in QuPath. Hypothesis testing was performed using generalized additive models (GAMMs) and generalized linear models (GLMs) to account for a multi-group, nested data structure.
    Slideview Vs200 Asw 4 1 Imaging System, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 3492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slideview+vs200+asw+4+1+imaging+system/VS200+Research+Slide+Scanner/pmc12687876-86-16-15
    Average 99 stars, based on 3492 article reviews
    slideview vs200 asw 4 1 imaging system - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Age-dependent remodelling of arterial chemoafferent innervation in hypertension"

    Article Title: Age-dependent remodelling of arterial chemoafferent innervation in hypertension

    Journal: Cardiovascular Research

    doi: 10.1093/cvr/cvaf207

    Study overview. Carotid bodies from SHR and Wistar controls were analysed across different age groups. The common carotid artery bifurcation (unilateral) was serially sectioned at 20 µm in the sagittal plane, with sections mounted in sequence (roman numerals on a representative slide) to permit reconstruction of rostral–caudal directionality following image acquisition. Multiplex fluorescent labelling targeted key components: chemosensory (glomus) cells (TH—tyrosine hydroxylase), vasculature (TL—tomato lectin), and innervation (NF—160 kDa neurofilament). Imaging was performed using a high-throughput widefield fluorescence microscopy platform (Olympus VS200). Lucy–Richardson deconvolution was applied, and binary area segmentation was performed using an artificial neural network-based pixel classification in QuPath. Hypothesis testing was performed using generalized additive models (GAMMs) and generalized linear models (GLMs) to account for a multi-group, nested data structure.
    Figure Legend Snippet: Study overview. Carotid bodies from SHR and Wistar controls were analysed across different age groups. The common carotid artery bifurcation (unilateral) was serially sectioned at 20 µm in the sagittal plane, with sections mounted in sequence (roman numerals on a representative slide) to permit reconstruction of rostral–caudal directionality following image acquisition. Multiplex fluorescent labelling targeted key components: chemosensory (glomus) cells (TH—tyrosine hydroxylase), vasculature (TL—tomato lectin), and innervation (NF—160 kDa neurofilament). Imaging was performed using a high-throughput widefield fluorescence microscopy platform (Olympus VS200). Lucy–Richardson deconvolution was applied, and binary area segmentation was performed using an artificial neural network-based pixel classification in QuPath. Hypothesis testing was performed using generalized additive models (GAMMs) and generalized linear models (GLMs) to account for a multi-group, nested data structure.

    Techniques Used: Sequencing, Multiplex Assay, Imaging, High Throughput Screening Assay, Fluorescence, Microscopy

    Related Articles

    Imaging:

    Article Title: Age-dependent remodelling of arterial chemoafferent innervation in hypertension
    Article Snippet: .. Prepared slides were imaged at the University of Auckland, Biomedical Imaging Research Unit, using an Olympus SLIDEVIEW VS200 ASW 4.1 imaging system equipped with a Hamamatsu ORCA-Flash4.0 V3 Digital CMOS camera. .. Multichannel images were acquired using fluorescence filter sets for DAPI (Ex: 365/20 nm; Em: 450/40 nm), FITC (490/20 nm; 525/36 nm), Cy3 (555/25 nm; 590/20 nm), and Cy5 (645/30 nm; 700/50 nm) powered by XYLIS XT720S LED illumination.

    Article Title: Age-dependent remodelling of arterial chemoafferent innervation in hypertension.
    Article Snippet: .. Prepared slides were imaged at the University of Auckland, Biomedical Imaging Research Unit 17 (BIRU) using an Olympus SLIDEVIEW VS200 ASW 4.1 imaging system equipped with 18 Hamamatsu ORCA-Flash4.0 V3 Digital CMOS camera. .. Multichannel images were acquired using 19 fluorescence filter sets for DAPI (Ex: 365/20 nm; Em: 450/40 nm), FITC (490/20 nm; 525/36 nm), 20 Cy3 (555/25 nm; 590/20 nm) and Cy5 (645/30 nm; 700/50 nm) powered by XYLIS XT720S LED 21 illumination.



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    Study overview. Carotid bodies from SHR and Wistar controls were analysed across different age groups. The common carotid artery bifurcation (unilateral) was serially sectioned at 20 µm in the sagittal plane, with sections mounted in sequence (roman numerals on a representative slide) to permit reconstruction of rostral–caudal directionality following image acquisition. Multiplex fluorescent labelling targeted key components: chemosensory (glomus) cells (TH—tyrosine hydroxylase), vasculature (TL—tomato lectin), and innervation (NF—160 kDa neurofilament). Imaging was performed using a high-throughput widefield fluorescence microscopy platform (Olympus <t>VS200).</t> Lucy–Richardson deconvolution was applied, and binary area segmentation was performed using an artificial neural network-based pixel classification in QuPath. Hypothesis testing was performed using generalized additive models (GAMMs) and generalized linear models (GLMs) to account for a multi-group, nested data structure.
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    Study overview. Carotid bodies from SHR and Wistar controls were analysed across different age groups. The common carotid artery bifurcation (unilateral) was serially sectioned at 20 µm in the sagittal plane, with sections mounted in sequence (roman numerals on a representative slide) to permit reconstruction of rostral–caudal directionality following image acquisition. Multiplex fluorescent labelling targeted key components: chemosensory (glomus) cells (TH—tyrosine hydroxylase), vasculature (TL—tomato lectin), and innervation (NF—160 kDa neurofilament). Imaging was performed using a high-throughput widefield fluorescence microscopy platform (Olympus <t>VS200).</t> Lucy–Richardson deconvolution was applied, and binary area segmentation was performed using an artificial neural network-based pixel classification in QuPath. Hypothesis testing was performed using generalized additive models (GAMMs) and generalized linear models (GLMs) to account for a multi-group, nested data structure.
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    Image Search Results


    Study overview. Carotid bodies from SHR and Wistar controls were analysed across different age groups. The common carotid artery bifurcation (unilateral) was serially sectioned at 20 µm in the sagittal plane, with sections mounted in sequence (roman numerals on a representative slide) to permit reconstruction of rostral–caudal directionality following image acquisition. Multiplex fluorescent labelling targeted key components: chemosensory (glomus) cells (TH—tyrosine hydroxylase), vasculature (TL—tomato lectin), and innervation (NF—160 kDa neurofilament). Imaging was performed using a high-throughput widefield fluorescence microscopy platform (Olympus VS200). Lucy–Richardson deconvolution was applied, and binary area segmentation was performed using an artificial neural network-based pixel classification in QuPath. Hypothesis testing was performed using generalized additive models (GAMMs) and generalized linear models (GLMs) to account for a multi-group, nested data structure.

    Journal: Cardiovascular Research

    Article Title: Age-dependent remodelling of arterial chemoafferent innervation in hypertension

    doi: 10.1093/cvr/cvaf207

    Figure Lengend Snippet: Study overview. Carotid bodies from SHR and Wistar controls were analysed across different age groups. The common carotid artery bifurcation (unilateral) was serially sectioned at 20 µm in the sagittal plane, with sections mounted in sequence (roman numerals on a representative slide) to permit reconstruction of rostral–caudal directionality following image acquisition. Multiplex fluorescent labelling targeted key components: chemosensory (glomus) cells (TH—tyrosine hydroxylase), vasculature (TL—tomato lectin), and innervation (NF—160 kDa neurofilament). Imaging was performed using a high-throughput widefield fluorescence microscopy platform (Olympus VS200). Lucy–Richardson deconvolution was applied, and binary area segmentation was performed using an artificial neural network-based pixel classification in QuPath. Hypothesis testing was performed using generalized additive models (GAMMs) and generalized linear models (GLMs) to account for a multi-group, nested data structure.

    Article Snippet: Prepared slides were imaged at the University of Auckland, Biomedical Imaging Research Unit, using an Olympus SLIDEVIEW VS200 ASW 4.1 imaging system equipped with a Hamamatsu ORCA-Flash4.0 V3 Digital CMOS camera.

    Techniques: Sequencing, Multiplex Assay, Imaging, High Throughput Screening Assay, Fluorescence, Microscopy